Today I could not finish “Protein Techniques.” Even not going to the University, I tried my best to go through the chapter. But many things disturbed me.
I like this book written by Garrett and Grisham and published by Thomson, Brooks/Cole. The authors are Biochemistry professors at the University of Virginia. If you read books authored by experienced scientists, you will learn a lot of things. I have pity over those persons in Science—Indians and Nepalis—who plagiarise things and sometimes just compiles chapters from different books, and pose themselves as learned authors.
I was talking about protein techniques. Let me recall some steps in the purification scheme of the macromolecule. With the pneumonic I have made.
Dialysis and Ultrafiltration (DU=Do)
Size Exclusion Chromatography (SexC=Sexc)
SDS-PAGE (SP== S pls)
Isoelectric Focusing (IF)
Two-Dimensional Gel Electrophoresis (2D)
Hydrophobic Interaction Chromatography (HIC)
High-Performance Liquid Chromatography (HPLC)
Affinity Chromatography (AC)
Do Sex E S-pls If 2D Hic High Ach Uc
I don’t want to make it more funny.